LOCUS       ECOLAC                  7477 bp    DNA     linear   BCT 05-MAY-1993
DEFINITION  E.coli lactose operon with lacI, lacZ, lacY and lacA genes.
ACCESSION   J01636 J01637 K01483 K01793
VERSION     J01636.1  GI:146575
KEYWORDS    acetyltransferase; beta-D-galactosidase; galactosidase; lac operon;
            lac repressor protein; lacA gene; lacI gene; lacY gene; lacZ gene;
            lactose permease; mutagenesis; palindrome; promoter region;
            thiogalactoside acetyltransferase.
SOURCE      Escherichia coli DNA; mRNA; clone lambda-h80dlac DNA; clone puk217;
            pgm8 (see comment).
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1243 to 1266)
  AUTHORS   Gilbert,W. and Maxam,A.
  TITLE     The nucleotide sequence of the lac operator
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 70, 3581-3584 (1973)
  MEDLINE   74055539
REFERENCE   2  (bases 1246 to 1308)
  AUTHORS   Maizels,N.M.
  TITLE     The nucleotide sequence of the lactose messenger ribonucleic acid
            transcribed from the UV5 promoter mutant of Escherichia coli
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 70, 3585-3589 (1973)
  MEDLINE   74055540
REFERENCE   3  (sites)
  AUTHORS   Gilbert,W., Maizels,N. and Maxam,A.
  TITLE     Sequences of controlling regions of the lactose operon
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 38, 845-855 (1974)
  MEDLINE   74174501
REFERENCE   4  (sites)
  AUTHORS   Gilbert,W., Gralla,J., Majors,A.J. and Maxam,A.
  TITLE     Lactose operator sequences and the action of lac repressor
  JOURNAL   (in) Sund,H. and Blauer,G. (Eds.);
            PROTEIN-LIGAND INTERACTIONS: 193-207;
            Walter de Gruyter, New York (1975)
REFERENCE   5  (bases 1146 to 1282)
  AUTHORS   Dickson,R.C., Abelson,J.N., Barnes,W.M. and Reznikoff,W.S.
  TITLE     Genetic regulation: The lac control region
  JOURNAL   Science 187, 27-35 (1975)
  MEDLINE   75048325
REFERENCE   6  (bases 1227 to 1271)
  AUTHORS   Gilbert,W., Maxam,A. and Mirzabekov,A.
  TITLE     Contacts between the lac repressor and DNA revealed by methylation
  JOURNAL   (in) Kjeldgaard,N.C. and Maaloe,O. (Eds.);
            CONTROL OF RIBOSOME SYNTHESIS: 138-143;
            Academic Press, New York (1976)
REFERENCE   7  (sites)
  AUTHORS   Marians,K.J. and Wu,R.
  TITLE     Structure of the lactose operator
  JOURNAL   Nature 260, 360-363 (1976)
  MEDLINE   76150089
REFERENCE   8  (bases 1242 to 1268)
  AUTHORS   Heyneker,H.L., Shine,J., Goodman,H.M., Boyer,H.W., Rosenberg,J.,
            Dickerson,R.E., Narang,S.A., Itakura,K., Lin,S. and Riggs,A.D.
  TITLE     Synthetic lac operator is functional in vivo
  JOURNAL   Nature 263, 748-752 (1976)
  MEDLINE   77056376
REFERENCE   9  (sites)
  AUTHORS   Dickson,R.C., Abelson,J.N., Johnson,P., Reznikoff,W.S. and
            Barnes,W.M.
  TITLE     Nucleotide sequence changes produced by mutations in the lac
            promoter of Escherichia coli
  JOURNAL   J. Mol. Biol. 111, 65-75 (1977)
  MEDLINE   77168230
REFERENCE   10 (bases 51 to 264)
  AUTHORS   Steege,D.A.
  TITLE     5'-terminal nucleotide sequence of Escherichia coli lactose
            repressor mRNA: Features of translational initiation and
            reinitiation sites
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 74, 4163-4167 (1977)
  MEDLINE   78052881
REFERENCE   11 (bases 1 to 81)
  AUTHORS   Calos,M.P.
  TITLE     DNA sequence for a low-level promoter of the lac repressor and an
            'up' promoter mutation
  JOURNAL   Nature 274, 762-765 (1978)
  MEDLINE   78246990
REFERENCE   12 (bases 49 to 1161)
  AUTHORS   Farabaugh,P.J.
  TITLE     Sequence of the lacI gene
  JOURNAL   Nature 274, 765-769 (1978)
  MEDLINE   78246991
REFERENCE   13 (sites)
  AUTHORS   Miller,J.H., Coulondre,C. and Farabaugh,P.J.
  TITLE     Correlation of nonsense sites in the lacI gene with specific codons
            in the nucleotide sequence
  JOURNAL   Nature 274, 770-775 (1978)
  MEDLINE   78246992
REFERENCE   14 (sites)
  AUTHORS   Calos,M.P. and Miller,J.H.
  TITLE     DNA sequence alteration resulting from a mutation impairing
            promoter function in the lac repressor gene
  JOURNAL   Mol. Gen. Genet. 178, 225-227 (1980)
  MEDLINE   80209248
REFERENCE   15 (bases 4306 to 5804)
  AUTHORS   Buechel,D.E., Gronenborn,B. and Mueller-Hill,B.
  TITLE     Sequence of the lactose permease gene
  JOURNAL   Nature 283, 541-545 (1980)
  MEDLINE   80120651
REFERENCE   16 (sites)
  AUTHORS   Miller,J.H., Calos,M.P. and Galas,D.J.
  TITLE     Genetic and sequencing studies of the specificity of transposition
            into the lac region of E. coli
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 45, 243-257 (1981)
  MEDLINE   82049502
REFERENCE   17 (sites)
  AUTHORS   Chenchick,A., Beabealashvilli,R.S. and Mirzabekov,A.
  TITLE     Topography of interaction of Escherichia coli RNA polymerase
            subunits with lac UV5 promoter
  JOURNAL   FEBS Lett. 128, 46-50 (1981)
  MEDLINE   82004657
REFERENCE   18 (sites)
  AUTHORS   Betz,J.L. and Sadler,J.R.
  TITLE     Variants of a cloned synthetic lactose operator: I. A palindromic
            dimer lactose operator derived from one strand of the cloned
            40-base pair operator
  JOURNAL   Gene 13, 1-12 (1981)
  MEDLINE   81213459
REFERENCE   19 (sites)
  AUTHORS   Sadler,J.R. and Tecklenburg,M.
  TITLE     Cloning and characterization of the natural lactose operator
  JOURNAL   Gene 13, 13-23 (1981)
  MEDLINE   81213463
REFERENCE   20 (sites)
  AUTHORS   Betz,J.L. and Sadler,J.R.
  TITLE     Variants of a cloned synthetic lactose operator: II.
            Chloramphenicol-resistant revertants retaining a lactose opeator in
            the CAT gene of plasmid pBR325
  JOURNAL   Gene 15, 187-200 (1981)
  MEDLINE   82051311
REFERENCE   21 (sites)
  AUTHORS   Calos,M.P. and Miller,J.H.
  TITLE     The DNA sequence change resulting from the I-Q1 mutation, which
            greatly increases promoter strength
  JOURNAL   Mol. Gen. Genet. 183, 559-560 (1981)
  MEDLINE   82147746
REFERENCE   22 (sites)
  AUTHORS   Mieschendahl,M., Buechel,D.E., Bocklage,H. and Mueller-Hill,B.
  TITLE     Mutations in the lacY gene of Escherichia coli define functional
            organization of lactose permease
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 78, 7652-7656 (1981)
  MEDLINE   82150928
REFERENCE   23 (sites)
  AUTHORS   Russell,D.R. and Bennett,G.N.
  TITLE     Construction and analysis of in vivo activity of E. coli promoter
            hybrids and promoter mutants that alter the -35 to -10 spacing
  JOURNAL   Gene 20, 231-243 (1982)
  MEDLINE   83158761
REFERENCE   24 (sites)
  AUTHORS   Horowitz,H. and Platt,T.
  TITLE     A termination site for lacI transcription is between the CAP site
            and the lac promoter
  JOURNAL   J. Biol. Chem. 257, 11740-11746 (1982)
  MEDLINE   83007251
REFERENCE   25 (sites)
  AUTHORS   Klein,R.D. and Wells,R.D.
  TITLE     Effects of neighboring DNA homopolymers on the biochemical and
            physical properties of the Escherichia coli lactose promoter: I.
            Cloning and characterization studies
  JOURNAL   J. Biol. Chem. 257, 12954-12961 (1982)
  MEDLINE   83030833
REFERENCE   26 (bases 1183 to 1291)
  AUTHORS   Weiher,H. and Schaller,H.
  TITLE     Segment-specific mutagenesis: Extensive mutagenesis of a lac
            promoter/operator element
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 79, 1408-1412 (1982)
  MEDLINE   82174608
REFERENCE   27 (sites)
  AUTHORS   Van Dyke,M.W. and Dervan,P.B.
  TITLE     Footprinting with MPE-Fe(II). Complementary-strand analyses of
            distamycin- and actinomycin-binding sites on heterogeneous DNA
  JOURNAL   Cold Spring Harb. Symp. Quant. Biol. 47, 347-353 (1983)
  MEDLINE   83233528
REFERENCE   28 (bases 1287 to 4364)
  AUTHORS   Kalnins,A., Otto,K., Ruether,U. and Mueller-Hill,B.
  TITLE     Sequence of the lacZ gene of Escherichia coli
  JOURNAL   EMBO J. 2, 593-597 (1983)
  MEDLINE   84028567
REFERENCE   29 (sites)
  AUTHORS   Cone,K.C., Sellitti,M.A. and Steege,D.A.
  TITLE     Lac repressor mRNA transcription terminates in vivo in the lac
            control region
  JOURNAL   J. Biol. Chem. 258, 11296-11304 (1983)
  MEDLINE   83291093
REFERENCE   30 (sites)
  AUTHORS   Sadler,J.R., Sasmor,H. and Betz,J.L.
  TITLE     A perfectly symmetric lac operator binds the lac repressor very
            tightly
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 80, 6785-6789 (1983)
  MEDLINE   84070714
REFERENCE   31 (sites)
  AUTHORS   Glickman,B.W. and Ripley,L.S.
  TITLE     Structural intermediates of deletion mutagenesis: A role for
            palindromic DNA
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 81, 512-516 (1984)
  MEDLINE   84119517
REFERENCE   32 (sites)
  AUTHORS   Spassky,A., Kirkegaard,K. and Buc,H.
  TITLE     Changes in the DNA structure of the lac UV5 promoter during
            formation of an open complex with Escherichia coli RNA polymerase
  JOURNAL   Biochemistry 24, 2723-2731 (1985)
  MEDLINE   85280412
REFERENCE   33 (sites)
  AUTHORS   Straney,D.C. and Crothers,D.M.
  TITLE     Intermediates in transcription initiation from the E. coli lac UV5
            promoter
  JOURNAL   Cell 43, 449-459 (1985)
  MEDLINE   86079527
REFERENCE   34 (sites)
  AUTHORS   Looman,A.C., de Gruyter,M., Vogelaar,A. and van Knippenberg,P.H.
  TITLE     Effects of heterologous ribosomal binding sites on the
            transcription and translation of the lacZ gene of Escherichia coli
  JOURNAL   Gene 37, 145-154 (1985)
  MEDLINE   86031346
REFERENCE   35 (sites)
  AUTHORS   Mandecki,W., Goldman,R.A., Powell,B.S. and Caruthers,M.H.
  TITLE     Lac up-promoter mutants with increased homology to the consensus
            promoter sequence
  JOURNAL   J. Bacteriol. 164, 1353-1355 (1985)
  MEDLINE   86059235
REFERENCE   36 (sites)
  AUTHORS   Malamy,M.H., Rahaim,P.T., Hoffman,C.S., Baghdoyan,D., O'Connor,M.B.
            and Miller,J.F.
  TITLE     A frameshift mutation at the junction of an IS1 insertion within
            lacZ restores beta-galactosidase activity via formation of an
            active lacZ-IS1 fusion protein
  JOURNAL   J. Mol. Biol. 181, 551-555 (1985)
  MEDLINE   85210885
REFERENCE   37 (bases 5646 to 7477)
  AUTHORS   Hediger,M.A., Johnson,D.F., Nierlich,D.P. and Zabin,I.
  TITLE     DNA sequence of the lactose operon: The lacA gene and the
            transcriptional termination region
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82, 6414-6418 (1985)
  MEDLINE   86016712
COMMENT     [3]  sites; UV5 mRNA transcripts and operator mutants. [(in)
            Sund,H. and Blauer,G. (eds.);Protein-Ligand Interactions:
            193-207;Walter de]  sites; operator mutational analysis. [7]
            sites; S1 and mung bean nuclease action on operator DNA. [9]
            sites; class I, II and III promoter mutant analysis. [13]  sites;
            lacI mutant analysis.
            [16]  sites; Tn5, Tn9 and Tn10 insertion sites in lac region. [14] 
            sites; lacI promoter mutation UJ177.
            [18]  sites; palindromic dimer operator;.
            [19]  sites; natural operator sequence.
            [20]  sites; operator mutational analysis.
            [21]  sites; lacI-Q deletion.
            [17]  sites; RNA polymerase UV5 promoter interaction. [22]  sites;
            lacY mutational analysis.
            [24]  sites; lacI-promoted transcription termination. [25]  sites;
            wt and UV5 promoter sequence studies. [23]  sites; UV5 promoter
            mutational analysis.
            [30]  sites; perfectly symmetric operator sequence. [29]  sites;
            lacI mRNA termination site.
            [27]  sites; distamycin and actinomycin binding to promoter. [31]
            sites; lacI deletion studies.
            [35]  sites; promoter mutational studies.
            [33]  sites; DNAase I studies with promoter sequence. [34]  sites;
            ribosomal binding and translation initiation for lacZ. [36]  sites;
            insertion sequence IS1 integration in lacZ;. [32]  sites; DNAase I
            studies with promoter.
            [1] first reports a 27 bp operator(sites 1240-1266) with two-fold
            symmetries; the operator has also been defined to be bases
            1246-1266 or bases 1239-1273 [8]. [(in) Kjeldgaard,N.C. and Maaloe,
            O.(eds);Control of ribosome synthesis: 138-143;A] explores the
            ability of lac
            repressor protein to affect methylation of operator DNA.  [8]
            argues that DNA on both sides of the 21 bp operator (bases
            1246-1266) affects repressor binding but that the sequences of this
            DNA are probably not critical. [5] gives a larger sequence known as
            the promoter-operator region for the wild-type, whereas [2] and
            [26] give portions of this region for the mutant strain UV5. Within
            the promoter region, bases 1162-1199 are identified as the
            catabolite gene activator protein binding site (cap) and bases
            1200-1245 are the RNA polymerase interaction site. [10] reports a
            sequence for the 5'end of the lacI (repressor) gene and discusses
            restart in mutant strains. [11] presents a sequence for the lacI
            promoter region and identifies an I-Q mutation which enhances lacI
            transcription approximately ten-fold. [12] gives a complete
            sequence
            for lacI which agrees with the known lac repressor sequence. [26]
            examines the promoter-operator region in the UV5 strain (lac109)
            and studies 23 mutant derivatives of this sequence. This sequence
            agrees with known protein sequences for the lacZ, lacY and lacA
            enzymes. [15] notes that the fMet codon is not present
            for lacA and suggests that the 'ttg' codon (5727-5729), which
            immediately precedes the mature N-terminal asparagine codon, is the
            start codon. The cds for lacZ, lacY and lacA are included on a
            single mRNA transcript.
            Complete source information:
            Escherichia coli DNA [1],[(in) Kjeldgaard,N.C. and Maaloe,O.(eds);
            Control of ribosome synthesis: 138-143;A],[8],[12],[26]; mRNA [2],
            [5],[10]; clone
            lambda-h80dlac DNA [11],[15]; clone puk217 [28]; pgm8 [37].
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     variation       16
                     /note="c in wild-type; t in 'up' promoter mutant I-Q [11]"
     mRNA            51..1230
                     /note="lacI (repressor) mRNA; preferred in vivo 3' end
                     [12],[29]"
     gene            79..1161
                     /gene="lacI"
     CDS             79..1161
                     /gene="lacI"
                     /note="lac repressor protein (gtg start codon)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24052.1"
                     /db_xref="GI:146576"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     misc_signal     1162..1199
                     /note="cap protein binding site"
     variation       1183..1186
                     /note="ttag in wild-type; aatt in strain UV5 [26]"
     variation       1209..1211
                     /note="gct in wild-type; gt in mutant l305 [5]"
     variation       1212
                     /note="t in wild-type; a in mutant l241 [5]"
     variation       1230
                     /note="c in wild-type; a in mutant p-r-1a [5]"
     variation       1237..1238
                     /note="gt in wild-type; aa in strain UV5 [26]"
     variation       1242..1245
                     /note="gtgg in wild-type; ttca in synthetic operator [8]"
     misc_signal     1246..1266
                     /note="lac repressor protein binding site"
     mRNA            1246..>4358
                     /note="lacZ mRNA [2],[5]"
     variation       1267..1268
                     /note="tc in wild-type; tg in synthetic operator [8]"
     variation       1282..1291
                     /note="ctatgaccat in wild-type; gatccggcca in strain UV5
                     [26]"
     gene            1284..4358
                     /gene="lacZ"
     CDS             1284..4358
                     /gene="lacZ"
                     /note="beta-d-galactosidase"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24053.1"
                     /db_xref="GI:146577"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            4410..5663
                     /gene="lacY"
     CDS             4410..5663
                     /gene="lacY"
                     /note="lactose permease"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24054.1"
                     /db_xref="GI:146578"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            5727..6338
                     /gene="lacA"
     CDS             5727..6338
                     /gene="lacA"
                     /note="thiogalactoside acetyltransferase (ttg start
                     codon)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA24055.1"
                     /db_xref="GI:551814"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
BASE COUNT     1739 a   1991 c   2004 g   1743 t
ORIGIN
       1  gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
      61  caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     121  gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     181  cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     241  caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     301  gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     361  gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     421  ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     481  gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     541  cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     601  gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     661  cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     721  gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     781  gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     841  cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     901  gataccgaag acagctcatg ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc
     961  ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
    1021  ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
    1081  caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    1141  cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    1201  accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    1261  acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
    1321  aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    1381  ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    1441  gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
    1501  gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
    1561  tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
    1621  cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
    1681  gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
    1741  tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
    1801  acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga
    1861  gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
    1921  tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
    1981  atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
    2041  actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
    2101  cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
    2161  gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
    2221  tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
    2281  tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
    2341  gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
    2401  tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
    2461  atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
    2521  aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
    2581  ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
    2641  gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
    2701  atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
    2761  acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
    2821  tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
    2881  cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
    2941  actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
    3001  atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
    3061  ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
    3121  cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
    3181  ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
    3241  tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
    3301  tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
    3361  ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
    3421  ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
    3481  ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
    3541  gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
    3601  ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
    3661  acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
    3721  cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
    3781  atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
    3841  gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
    3901  tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
    3961  cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
    4021  ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
    4081  cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
    4141  gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
    4201  atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
    4261  ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg
    4321  gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc
    4381  tgcccgtatt tcgcgtaagg aaatccatta tgtactattt aaaaaacaca aacttttgga
    4441  tgttcggttt attctttttc ttttactttt ttatcatggg agcctacttc ccgtttttcc
    4501  cgatttggct acatgacatc aaccatatca gcaaaagtga tacgggtatt atttttgccg
    4561  ctatttctct gttctcgcta ttattccaac cgctgtttgg tctgctttct gacaaactcg
    4621  ggctgcgcaa atacctgctg tggattatta ccggcatgtt agtgatgttt gcgccgttct
    4681  ttatttttat cttcgggcca ctgttacaat acaacatttt agtaggatcg attgttggtg
    4741  gtatttatct aggcttttgt tttaacgccg gtgcgccagc agtagaggca tttattgaga
    4801  aagtcagccg tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct
    4861  gggcgctgtg tgcctcgatt gtcggcatca tgttcaccat caataatcag tttgttttct
    4921  ggctgggctc tggctgtgca ctcatcctcg ccgttttact ctttttcgcc aaaacggatg
    4981  cgccctcttc tgccacggtt gccaatgcgg taggtgccaa ccattcggca tttagcctta
    5041  agctggcact ggaactgttc agacagccaa aactgtggtt tttgtcactg tatgttattg
    5101  gcgtttcctg cacctacgat gtttttgacc aacagtttgc taatttcttt acttcgttct
    5161  ttgctaccgg tgaacagggt acgcgggtat ttggctacgt aacgacaatg ggcgaattac
    5221  ttaacgcctc gattatgttc tttgcgccac tgatcattaa tcgcatcggt gggaaaaacg
    5281  ccctgctgct ggctggcact attatgtctg tacgtattat tggctcatcg ttcgccacct
    5341  cagcgctgga agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg
    5401  tgggctgctt taaatatatt accagccagt ttgaagtgcg tttttcagcg acgatttatc
    5461  tggtctgttt ctgcttcttt aagcaactgg cgatgatttt tatgtctgta ctggcgggca
    5521  atatgtatga aagcatcggt ttccagggcg cttatctggt gctgggtctg gtggcgctgg
    5581  gcttcacctt aatttccgtg ttcacgctta gcggccccgg cccgctttcc ctgctgcgtc
    5641  gtcaggtgaa tgaagtcgct taagcaatca atgtcggatg cggcgcgacg cttatccgac
    5701  caacatatca taacggagtg atcgcattga acatgccaat gaccgaaaga ataagagcag
    5761  gcaagctatt taccgatatg tgcgaaggct taccggaaaa aagacttcgt gggaaaacgt
    5821  taatgtatga gtttaatcac tcgcatccat cagaagttga aaaaagagaa agcctgatta
    5881  aagaaatgtt tgccacggta ggggaaaacg cctgggtaga accgcctgtc tatttctctt
    5941  acggttccaa catccatata ggccgcaatt tttatgcaaa tttcaattta accattgtcg
    6001  atgactacac ggtaacaatc ggtgataacg tactgattgc acccaacgtt actctttccg
    6061  ttacgggaca ccctgtacac catgaattga gaaaaaacgg cgagatgtac tcttttccga
    6121  taacgattgg caataacgtc tggatcggaa gtcatgtggt tattaatcca ggcgtcacca
    6181  tcggggataa ttctgttatt ggcgcgggta gtatcgtcac aaaagacatt ccaccaaacg
    6241  tcgtggcggc tggcgttcct tgtcgggtta ttcgcgaaat aaacgaccgg gataagcact
    6301  attatttcaa agattataaa gttgaatcgt cagtttaaat tataaaaatt gcctgatacg
    6361  ctgcgcttat caggcctaca agttcagcga tctacattag ccgcatccgg catgaacaaa
    6421  gcgcaggaac aagcgtcgca tcatgcctct ttgacccaca gctgcggaaa acgtactggt
    6481  gcaaaacgca gggttatgat catcagccca acgacgcaca gcgcatgaaa tgcccagtcc
    6541  atcaggtaat tgccgctgat actacgcagc acgccagaaa accacggggc aagcccggcg
    6601  atgataaaac cgattccctg cataaacgcc accagcttgc cagcaatagc cggttgcaca
    6661  gagtgatcga gcgccagcag caaacagagc ggaaacgcgc cgcccagacc taacccacac
    6721  accatcgccc acaataccgg caattgcatc ggcagccaga taaagccgca gaaccccacc
    6781  agttgtaaca ccagcgccag cattaacagt ttgcgccgat cctgatggcg agccatagca
    6841  ggcatcagca aagctcctgc ggcttgccca agcgtcatca atgccagtaa ggaaccgctg
    6901  tactgcgcgc tggcaccaat ctcaatatag aaagcgggta accaggcaat caggctggcg
    6961  taaccgccgt taatcagacc gaagtaaaca cccagcgtcc acgcgcgggg agtgaatacc
    7021  acgcgaaccg gagtggttgt tgtcttgtgg gaagaggcga cctcgcgggc gctttgccac
    7081  caccaggcaa agagcgcaac aacggcaggc agcgccacca ggcgagtgtt tgataccagg
    7141  tttcgctatg ttgaactaac cagggcgtta tggcggcacc aagcccaccg ccgcccatca
    7201  gagccgcgga ccacagcccc atcaccagtg gcgtgcgctg ctgaaaccgc cgtttaatca
    7261  ccgaagcatc accgcctgaa tgatgccgat ccccacccca ccaagcagtg cgctgctaag
    7321  cagcagcgca ctttgcgggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag
    7381  actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc
    7441  cagcccgccc atggtaacca ccggcagagc ggtcgac
//
LOCUS       ECLACA                  1832 bp    DNA     linear   BCT 05-JUL-1999
DEFINITION  Escherichia coli lacA gene for thiogalactoside transacetylase.
ACCESSION   X51872
VERSION     X51872.1  GI:41891
KEYWORDS    lac operon; lacA gene; lacY gene; thiogalactoside transacetylase.
SOURCE      Escherichia coli.
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Hediger,M.A., Johnson,D.F., Nierlich,D.P. and Zabin,I.
  TITLE     DNA sequence of the lactose operon: The lacA gene and the
            transcriptional termination region
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82, 6414-6418 (1985)
  MEDLINE   86016712
  REMARK    (1-1832)
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     CDS             <1..18
                     /codon_start=1
                     /transl_table=11
                     /product="lacY gene product"
                     /protein_id="CAA36161.1"
                     /db_xref="GI:4377496"
                     /translation="VNEVA"
     gene            82..693
                     /gene="lacA"
     CDS             82..693
                     /gene="lacA"
                     /codon_start=1
                     /transl_table=11
                     /product="thiogalactoside transacetylase"
                     /protein_id="CAA36162.1"
                     /db_xref="GI:581122"
                     /db_xref="SWISS-PROT:P07464"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
BASE COUNT      519 a    510 c    450 g    353 t
ORIGIN
       1  gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg cgacgcttat ccgaccaaca
      61  tatcataacg gagtgatcgc attgaacatg ccaatgaccg aaagaataag agcaggcaag
     121  ctatttaccg atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa aacgttaatg
     181  tatgagttta atcactcgca tccatcagaa gttgaaaaaa gagaaagcct gattaaagaa
     241  atgtttgcca cggtagggga aaacgcctgg gtagaaccgc ctgtctattt ctcttacggt
     301  tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat tgtcgatgac
     361  tacacggtaa caatcggtga taacgtactg attgcaccca acgttactct ttccgttacg
     421  ggacaccctg tacaccatga attgagaaaa aacggcgaga tgtactcttt tccgataacg
     481  attggcaata acgtctggat cggaagtcat gtggttatta atccaggcgt caccatcggg
     541  gataattctg ttattggcgc gggtagtatc gtcacaaaag acattccacc aaacgtcgtg
     601  gcggctggcg ttccttgtcg ggttattcgc gaaataaacg accgggataa gcactattat
     661  ttcaaagatt ataaagttga atcgtcagtt taaattataa aaattgcctg atacgctgcg
     721  cttatcaggc ctacaagttc agcgatctac attagccgca tccggcatga acaaagcgca
     781  ggaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgta ctggtgcaaa
     841  acgcagggtt atgatcatca gcccaacgac gcacagcgca tgaaatgccc agtccatcag
     901  gtaattgccg ctgatactac gcagcacgcc agaaaaccac ggggcaagcc cggcgatgat
     961  aaaaccgatt ccctgcataa acgccaccag cttgccagca atagccggtt gcacagagtg
    1021  atcgagcgcc agcagcaaac agagcggaaa cgcgccgccc agacctaacc cacacaccat
    1081  cgcccacaat accggcaatt gcatcggcag ccagataaag ccgcagaacc ccaccagttg
    1141  taacaccagc gccagcatta acagtttgcg ccgatcctga tggcgagcca tagcaggcat
    1201  cagcaaagct cctgcggctt gcccaagcgt catcaatgcc agtaaggaac cgctgtactg
    1261  cgcgctggca ccaatctcaa tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc
    1321  gccgttaatc agaccgaagt aaacacccag cgtccacgcg cggggagtga ataccacgcg
    1381  aaccggagtg gttgttgtct tgtgggaaga ggcgacctcg cgggcgcttt gccaccacca
    1441  ggcaaagagc gcaacaacgg caggcagcgc caccaggcga gtgtttgata ccaggtttcg
    1501  ctatgttgaa ctaaccaggg cgttatggcg gcaccaagcc caccgccgcc catcagagcc
    1561  gcggaccaca gccccatcac cagtggcgtg cgctgctgaa accgccgttt aatcaccgaa
    1621  gcatcaccgc ctgaatgatg ccgatcccca ccccaccaag cagtgcgctg ctaagcagca
    1681  gcgcactttg cgggtaaagc tcacgcatca atgcaccgac ggcaatcagc aacagactga
    1741  tggcgacact gcgacgttcg ctgacatgct gatgaagcca gcttccggcc agcgccagcc
    1801  cgcccatggt aaccaccggc agagcggtcg ac
//
LOCUS       ECLACI                  1113 bp    DNA     linear   BCT 10-FEB-1999
DEFINITION  E. coli laci gene (codes for the lac repressor).
ACCESSION   V00294
VERSION     V00294.1  GI:41893
KEYWORDS    DNA binding protein; repressor.
SOURCE      Escherichia coli.
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Farabaugh,P.J.
  TITLE     Sequence of the lacI gene
  JOURNAL   Nature 274 (5673), 765-769 (1978)
  MEDLINE   78246991
COMMENT     KST ECO.LACI.
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     CDS             31..1113
                     /note="reading frame"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23569.1"
                     /db_xref="GI:581123"
                     /db_xref="SWISS-PROT:P03023"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
BASE COUNT      249 a    304 c    322 g    238 t
ORIGIN
       1  ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca
      61  gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt
     121  tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
     181  cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
     241  ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
     301  ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
     361  gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
     421  caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
     481  tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
     541  gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
     601  tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
     661  cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
     721  caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
     781  ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
     841  gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac caccatcaaa
     901  caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     961  caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
    1021  gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    1081  cgacaggttt cccgactgga aagcgggcag tga
//
LOCUS       ECLACY                  1500 bp    DNA     linear   BCT 07-JUL-1995
DEFINITION  E. coli lacY gene (codes for lactose permease).
ACCESSION   V00295
VERSION     V00295.1  GI:41897
KEYWORDS    membrane protein.
SOURCE      Escherichia coli.
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Buchel,D.E., Gronenborn,B. and Muller-Hill,B.
  TITLE     Sequence of the lactose permease gene
  JOURNAL   Nature 283 (5747), 541-545 (1980)
  MEDLINE   80120651
COMMENT     lacZ is a beta-galactosidase and lacA is transacetylase. KST
            ECO.LACY.
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     CDS             <1..54
                     /note="reading frame (lacZ)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23570.1"
                     /db_xref="GI:41898"
                     /db_xref="SWISS-PROT:P00722"
                     /translation="FQLSAGRYHYQLVWCQK"
     CDS             106..1359
                     /note="reading frame (lacY)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23571.1"
                     /db_xref="GI:41899"
                     /db_xref="SWISS-PROT:P02920"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     CDS             1423..>1500
                     /note="reading frame (lacA)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23572.1"
                     /db_xref="GI:581124"
                     /db_xref="SWISS-PROT:P07464"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKR"
BASE COUNT      315 a    342 c    357 g    486 t
ORIGIN
       1  ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa
      61  ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa
     121  aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc
     181  tacttcccgt ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg
     241  ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg
     301  ctttctgaca aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg
     361  atgtttgcgc cgttctttat ttttatcttc gggccactgt tacaatacaa cattttagta
     421  ggatcgattg ttggtggtat ttatctaggc ttttgtttta acgccggtgc gccagcagta
     481  gaggcattta ttgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg
     541  tttggctgtg ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat
     601  aatcagtttg ttttctggct gggctctggc tgtgcactca tcctcgccgt tttactcttt
     661  ttcgccaaaa cggatgcgcc ctcttctgcc acggttgcca atgcggtagg tgccaaccat
     721  tcggcattta gccttaagct ggcactggaa ctgttcagac agccaaaact gtggtttttg
     781  tcactgtatg ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat
     841  ttctttactt cgttctttgc taccggtgaa cagggtacgc gggtatttgg ctacgtaacg
     901  acaatgggcg aattacttaa cgcctcgatt atgttctttg cgccactgat cattaatcgc
     961  atcggtggga aaaacgccct gctgctggct ggcactatta tgtctgtacg tattattggc
    1021  tcatcgttcg ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa
    1081  gtaccgttcc tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt
    1141  tcagcgacga tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg
    1201  tctgtactgg cgggcaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg
    1261  ggtctggtgg cgctgggctt caccttaatt tccgtgttca cgcttagcgg ccccggcccg
    1321  ctttccctgc tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc
    1381  gcgacgctta tccgaccaac atatcataac ggagtgatcg cattgaacat gccaatgacc
    1441  gaaagaataa gagcaggcaa gctatttacc gatatgtgcg aaggcttacc ggaaaaaaga
//
LOCUS       ECLACZ                  3078 bp    DNA     linear   BCT 06-MAR-1996
DEFINITION  E. coli gene lacZ coding for beta-galactosidase (EC 3.2.1.23).
ACCESSION   V00296
VERSION     V00296.1  GI:41901
KEYWORDS    galactosidase.
SOURCE      Escherichia coli.
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Kalnins,A., Otto,K., Ruther,U. and Muller-Hill,B.
  TITLE     Sequence of the lacZ gene of Escherichia coli
  JOURNAL   EMBO J. 2 (4), 593-597 (1983)
  MEDLINE   84028567
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Zell,R. and Fritz,H.J.
  TITLE     DNA mismatch-repair in Escherichia coli counteracting the
            hydrolytic deamination of 5-methyl-cytosine residues
  JOURNAL   EMBO J. 6 (6), 1809-1815 (1987)
  MEDLINE   87275876
COMMENT     Data kindly reviewed (18-MAY-1983) by U. Ruether.
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     mat_peptide     1..3069
                     /product="unnamed"
     CDS             <1..3072
                     /note="galactosidase"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA23573.1"
                     /db_xref="GI:1197203"
                     /db_xref="SWISS-PROT:P00722"
                     /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTN
                     VTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVG
                     YGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKP
                     TTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGG
                     EIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFRE
                     VRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHY
                     PNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNH
                     PSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDE
                     DQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGF
                     VWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQ
                     QFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIEL
                     PELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHL
                     TTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEA
                     TRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTY
                     RIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAAC
                     FDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSH
                     RHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
BASE COUNT      682 a    841 c    886 g    669 t
ORIGIN
       1  accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      61  gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     121  gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
     181  gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
     241  gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
     301  accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
     361  ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
     421  attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
     481  tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
     541  gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag
     601  gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
     661  caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
     721  gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
     781  tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     841  gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     901  tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     961  acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
    1021  ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
    1081  cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
    1141  cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
    1201  ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
    1261  gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
    1321  acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
    1381  gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
    1441  tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
    1501  ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
    1561  aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
    1621  gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
    1681  cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
    1741  aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
    1801  ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
    1861  aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
    1921  gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
    1981  aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
    2041  gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
    2101  gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
    2161  cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
    2221  accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
    2281  cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
    2341  cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
    2401  attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
    2461  gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
    2521  gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
    2581  agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
    2641  cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
    2701  gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
    2761  ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
    2821  acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
    2881  cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
    2941  ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
    3001  ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg
    3061  tgtcaaaaat aataataa
//
LOCUS       PAAMIB                  1212 bp    DNA     linear   BCT 10-AUG-1995
DEFINITION  P.aeruginosa (PAC1) amiB gene.
ACCESSION   X77160
VERSION     X77160.1  GI:473108
KEYWORDS    amiB gene.
SOURCE      Pseudomonas aeruginosa.
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; gamma subdivision; Pseudomonadaceae;
            Pseudomonas.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Wilson,S.A., Wachira,S.M., Drew,R.E., Jones,D. and Pearl,L.H.
  TITLE     Identification of a putative ABC transporter in the Pseudomonas
            aeruginosa amidase operon
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Wilson,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-1994) S.A. Wilson, University College London,
            Dept of Biochemistry, Gower Street, London WC1E 6BT, UK
REFERENCE   3  (bases 1 to 1212)
  AUTHORS   Wilson,S.A., Williams,R.J., Pearl,L.H. and Drew,R.E.
  TITLE     Identification of two new genes in the Pseudomonas aeruginosa
            amidase operon, encoding an ATPase (AmiB) and a putative integral
            membrane protein (AmiS)
  JOURNAL   J. Biol. Chem. 270 (32), 18818-18824 (1995)
  MEDLINE   95370186
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Pseudomonas aeruginosa"
                     /strain="PAC1"
                     /db_xref="taxon:287"
                     /clone="plasmid pAS20"
     gene            97..1212
                     /gene="amiB"
     CDS             97..1212
                     /gene="amiB"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA54405.1"
                     /db_xref="GI:473109"
                     /db_xref="SWISS-PROT:Q51416"
                     /translation="MPFLSDMLDQSRRQQDEEQALARENLAEASLLQAHLSHRSALHS
                     RFRFDPAAVMDCLRAEVLGQEPALQAVEDMLKVVRADIADPRRPLFSALFLGPTGVGK
                     TEIVRALARALHGDAEGFCRVDMNTLSQEHYAAALTGAPPGYVGAKEGTTLLEQDKLD
                     GSPGRPGIVLFDELEKASPEVVHALLNVLDNGLLRVASGERTYHFRNTLVFMTSNLCA
                     HEIQRYDERRQRLPWRLLPVGGERRRRDIDGMVRARLLKTFSPEFVNRLDSVVTFNWI
                     ERDVVARLVELEVQRLNRRLEKHRCRLEATPEVLAKIARAGFDRQFGARALRRSVRHH
                     LEVPLAEHLLDHHQPGDGNCTIYLASLEHERVRFVRR"
BASE COUNT      193 a    426 c    403 g    190 t
ORIGIN
       1  gaaggaagcc tgacggcaga cgccgccagc ccggcgtgcc gtcgtgcggc acgccgtctc
      61  cgccatttcc cccctgtggc agaaggagtt tcatccatgc cttttctgag cgacatgctc
     121  gaccagtccc gccggcagca ggacgaggaa caggccctgg cgcgggagaa tcttgccgag
     181  gcaagtctgc tccaggccca cctgagtcac cgcagcgccc tgcacagccg tttccgtttc
     241  gacccggcgg cggtgatgga ctgcctgcgc gccgaggtgc tcggccagga accggcgcta
     301  caggccgtcg aggacatgct caaggtggtt cgcgcggaca tcgccgaccc gcgccgtccg
     361  ctgttcagcg cgctgttcct cggccccacc ggagtcggca agaccgagat cgtgcgcgcc
     421  ctggccaggg ccctgcacgg cgacgccgag gggttctgcc gggtggacat gaacaccctg
     481  tcccaggagc actatgccgc cgccctcacc ggtgcgccgc cgggctacgt cggggcgaag
     541  gagggcacca ccctgttgga gcaggacaag ctggacggca gtcccgggcg ccccggcatc
     601  gttctcttcg acgaactgga aaaggccagc ccggaagtgg tccatgcgtt gctcaacgta
     661  ctcgacaacg gcctgctacg ggtcgcttcc ggcgaacgca cctaccattt ccgcaacacc
     721  ctggtgttca tgaccagcaa tctctgcgcc catgagatcc agcgctacga cgagcgtcgc
     781  cagcgcctgc cctggcgcct gctgccggtc ggcggcgagc gccggcggcg ggacatcgac
     841  gggatggtcc gggcccggct gctgaagacc ttctcgccgg agttcgtcaa tcgtctcgat
     901  agcgtggtca ccttcaactg gatcgaacgc gacgtcgtcg cgcgcctggt cgagctggag
     961  gtgcagcggc tcaaccggcg cctggagaag catcgctgcc gcctggaggc gaccccggag
    1021  gtgctggcga agatcgcccg cgccggcttc gaccggcagt tcggcgcccg tgcgttgcgc
    1081  cgcagcgtcc gtcatcatct cgaggtaccg ctggccgagc atctgctcga tcaccaccag
    1141  ccgggcgacg ggaactgcac gatctacctg gcgagcctgg agcacgagcg ggttcgcttc
    1201  gtacggcgct ga
//
LOCUS       PSEAIME                 1065 bp    DNA     linear   BCT 26-APR-1993
DEFINITION  P.aeruginosa aimE gene encoding alphatic amidase, complete cds.
ACCESSION   M27612
VERSION     M27612.1  GI:150979
KEYWORDS    alphatic amidase.
SOURCE      P.aeruginosa (strain PAC433) DNA.
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; gamma subdivision; Pseudomonadaceae;
            Pseudomonas.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Brammar,W.J., Charles,I.G., Matfield,M., Cheng-Pin,L., Drew,R.E.
            and Clarke,P.H.
  TITLE     The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa
  JOURNAL   FEBS Lett. 215, 291-294 (1987)
  MEDLINE   87219102
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Pseudomonas aeruginosa"
                     /db_xref="taxon:287"
     CDS             25..1065
                     /note="alphatic amidase (EC 3.5.1.4)"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="AAA25697.1"
                     /db_xref="GI:150980"
                     /translation="MRHGDISSSNDTVGVAVVNYKMPRLHTAAEVLDNARKIADMIVG
                     MKQGLPGMDLVVFPEYSLQGIMYDPAEMMETAVAIPGEETEIFSRACRKANVWGVFSL
                     TGERHEEHPRKAPYNTLVLIDNNGEIVQKYRKIIPWCPIEGWYPGGQTYVSEGPKGMK
                     ISLIICDDPNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVMMAKAMAWANNCYVAVA
                     NAAGFDGVYSYFGHSAIIGFDGRTLGECGEEEMGIQYAQLSLSQIRDARANDQSQNHL
                     FKILHRGYSGLQASGDGDRGLAECPFEFYRTWVTDAEKARDNVERLTRSTTGVAQCPV
                     GRLPYEGLEKEA"
BASE COUNT      220 a    337 c    343 g    165 t
ORIGIN
       1  aaataacaac aggaggtgat acccatgcgt cacggcgata tttccagcag caacgacacc
      61  gtcggagtgg ccgtggtcaa ctacaagatg ccgcgcctgc acaccgcggc ggaggtcctg
     121  gacaacgccc ggaagatcgc cgacatgatc gtcggcatga agcagggcct gcccggcatg
     181  gacctggtgg tgttcccgga gtacagcctg cagggcatca tgtacgatcc ggcggagatg
     241  atggaaaccg cggtggcgat ccccggcgag gaaaccgaga tattctcccg cgcctgccgc
     301  aaggccaacg tctggggcgt attctccctc accggcgaac ggcacgagga gcatccgcgc
     361  aaggcgccgt acaacaccct ggtgctgatc gacaacaacg gcgagatcgt ccagaagtac
     421  cgcaagatca ttccctggtg ccccatcgag ggctggtatc ccggtggcca gacctacgtc
     481  agcgaagggc cgaagggcat gaagatcagc ctgatcatct gcgacgaccc caattacccg
     541  gagatctggc gcgactgcgc gatgaagggc gccgagctga tcgtgcgctg ccagggctac
     601  atgtacccgg ccaaggacca gcaggtgatg atggccaagg ccatggcctg ggccaacaac
     661  tgctatgtgg cggtggccaa cgcggccggc ttcgacggtg tctattccta cttcggccac
     721  tcggcgatca tcggcttcga cggccgtacc ctcggtgagt gcggcgagga ggaaatgggt
     781  atccagtacg cccagctgtc cctttcgcag atccgcgatg cgcgcgccaa cgatcagtcg
     841  cagaaccacc tgttcaagat cctccaccgc ggctacagcg gcttgcaggc gtccggcgac
     901  ggcgaccggg gcctggcgga gtgtccgttc gagttctacc gcacctgggt caccgacgcc
     961  gagaaggcgc gcgacaatgt cgagcgactg acccgctcga ccaccggcgt ggcgcaatgc
    1021  ccggtcggcc ggctgcccta tgagggactg gagaaggaag cctga
//
LOCUS       PAAMIR                  2167 bp    DNA     linear   BCT 17-FEB-1997
DEFINITION  Pseudomonas aeruginosa amiC and amiR gene for aliphatic amidase
            regulation.
ACCESSION   X13776 M43175
VERSION     X13776.1  GI:45269
KEYWORDS    aliphatic amidase regulator; amiC gene; amiR gene.
SOURCE      Pseudomonas aeruginosa.
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; gamma subdivision; Pseudomonadaceae;
            Pseudomonas.
REFERENCE   1  (bases 1167 to 2167)
  AUTHORS   Rice,P.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-DEC-1988) Rice P.M., EMBL, Postfach 10-2209,
            Meyerhofstrasse 1, 6900 Heidelberg, FRG
REFERENCE   2  (bases 1167 to 2167)
  AUTHORS   Lowe,N., Rice,P.M. and Drew,R.E.
  TITLE     Nucleotide sequence of the aliphatic amidase regulator gene (amiR)
            of Pseudomonas aeruginosa
  JOURNAL   FEBS Lett. 246 (1-2), 39-43 (1989)
  MEDLINE   89211409
REFERENCE   3  (bases 1 to 1292)
  AUTHORS   Wilson,S. and Drew,R.
  TITLE     Cloning and DNA sequence of amiC, a new gene regulating expression
            of the Pseudomonas aeruginosa aliphatic amidase, and purification
            of the amiC product
  JOURNAL   J. Bacteriol. 173 (16), 4914-4921 (1991)
  MEDLINE   91317707
REFERENCE   4  (bases 1 to 2167)
  AUTHORS   Rice,P.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-1991) Rice P.M., EMBL, Postfach 10-2209,
            Meyerhofstrasse 1, 6900 Heidelberg, FRG
COMMENT     Data kindly reviewed (04-SEP-1991) by Rice P. M.
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Pseudomonas aeruginosa"
                     /strain="PAC"
                     /isolate="PAC 1"
                     /db_xref="taxon:287"
                     /map="38 min"
     misc_feature    1
                     /note="last base of an XhoI site"
     promoter        8..24
                     /note="proposed rpoN-dependent promoter"
     promoter        65..81
                     /note="proposed rpoN-dependent promoter"
     RBS             121..126
                     /note="proposed Shine-Dalgarno sequence"
     gene            135..1292
                     /gene="amiC"
     CDS             135..1292
                     /gene="amiC"
                     /note="negative regulator of amiR"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA32024.1"
                     /db_xref="GI:45271"
                     /db_xref="SWISS-PROT:P27017"
                     /translation="MGSHQERPLIGLLFSETGVTADIERSHAYGALLAVEQLNREGGV
                     GGRPIETLSQDPGGDPDRYRLCAEDFIRNRGVRFLVGCYMSHTRKAVMPVVERADALL
                     CYPTPYEGFEYSPNIVYGGPAPNQNSAPLAAYLIRHYGERVVFIGSDYIYPRESNHVM
                     RHLYRQHGGTVLEEIYIPLYPSDDDLQRAVERIYQARADVVFSTVVGTGTAELYRAIA
                     RRYGDGRRPPIASLTTSEAEVAKMESDVAEGQVVVAPYFSSIDTPASRAFVQACHGFF
                     PENATITAWAEAAYWQTLLLGRAAQAAGNWRVEDVQRHLYDIDIDAPQGPVRVERQNN
                     HSRLSSRIAEIDARGVFQVRWQSPEPIRPDPYVVVHNLDDWSASMGGGPLP"
     misc_feature    648..653
                     /gene="amiC"
                     /note="end of 658bp XhoI fragment, deletion in  pSW3
                     causes constitutive expression of amiE"
     variation       912..1167
                     /gene="amiC"
                     /note="ClaI fragment deleted in pSW36,  constitutive
                     phenotype"
                     /replace=""
     conflict        1281
                     /gene="amiC"
                     /citation=[3]
                     /replace="g"
     gene            1289..1879
                     /gene="amiR"
     CDS             1289..1879
                     /gene="amiR"
                     /note="aliphatic amidase regulator, positive  regulator of
                     amiE"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA32023.1"
                     /db_xref="GI:45270"
                     /db_xref="SWISS-PROT:P10932"
                     /translation="MSANSLLGSLRELQVLVLNPPGEVSDALVLQLIRIGCSVRQCWP
                     PPEAFDVPVDVVFTSIFQNGHHDEIAALLAAGTPRTTLVALVEYESPAVLSQIIELEC
                     HGVITQPLDAHRVLPVLVSARRISEEMAKLKQKTEQLQDRIAGQARINQAKVLLMQRH
                     GWDEREAHQHLSREAMKRREPILKIAQELLGNEPSA"
BASE COUNT      363 a    712 c    730 g    362 t
ORIGIN
       1  ggtaccgctg gccgagcatc tgctcgatca ccaccagccg ggcgacggga actgcacgat
      61  ctacctggcg agcctggagc acgagcgggt tcgcttcgta cggcgctgag cgacagtcac
     121  aggagaggaa acggatggga tcgcaccagg agcggccgct gatcggcctg ctgttctccg
     181  aaaccggcgt caccgccgat atcgagcgct cgcacgcgta tggcgcattg ctcgcggtcg
     241  agcaactgaa ccgcgagggc ggcgtcggcg gtcgcccgat cgaaacgctg tcccaggacc
     301  ccggcggcga cccggaccgc tatcggctgt gcgccgagga cttcattcgc aaccgggggg
     361  tacggttcct cgtgggctgc tacatgtcgc acacgcgcaa ggcggtgatg ccggtggtcg
     421  agcgcgccga cgcgctgctc tgctacccga ccccctacga gggcttcgag tattcgccga
     481  acatcgtcta cggcggtccg gcgccgaacc agaacagtgc gccgctggcg gcgtacctga
     541  ttcgccacta cggcgagcgg gtggtgttca tcggctcgga ctacatctat ccgcgggaaa
     601  gcaaccatgt gatgcgccac ctgtatcgcc agcacggcgg cacggtgctc gaggaaatct
     661  acattccgct gtatccctcc gacgacgact tgcagcgcgc cgtcgagcgc atctaccagg
     721  cgcgcgccga cgtggtcttc tccaccgtgg tgggcaccgg caccgccgag ctgtatcgcg
     781  ccatcgcccg tcgctacggc gacggcaggc ggccgccgat cgccagcctg accaccagcg
     841  aggcggaggt ggcgaagatg gagagtgacg tggcagaggg gcaggtggtg gtcgcgcctt
     901  acttctccag catcgatacg cccgccagcc gggccttcgt ccaggcctgc catggtttct
     961  tcccggagaa cgcgaccatc accgcctggg ccgaggcggc ctactggcag accttgttgc
    1021  tcggccgcgc cgcgcaggcc gcaggcaact ggcgggtgga agacgtgcag cggcacctgt
    1081  acgacatcga catcgacgcg ccacaggggc cggtccgggt ggagcgccag aacaaccaca
    1141  gccgcctgtc ttcgcgcatc gcggaaatcg atgcgcgcgg cgtgttccag gtccgctggc
    1201  agtcgcccga accgattcgc cccgaccctt atgtcgtcgt gcataacctc gacgactggt
    1261  ccgccagcat gggcggggga ccgctcccat gagcgccaac tcgctgctcg gcagcctgcg
    1321  cgagttgcag gtgctggtcc tcaacccgcc gggggaggtc agcgacgccc tggtcttgca
    1381  gctgatccgc atcggttgtt cggtgcgcca gtgctggccg ccgccggaag ccttcgacgt
    1441  gccggtggac gtggtcttca ccagcatttt ccagaatggc caccacgacg agatcgctgc
    1501  gctgctcgcc gccgggactc cgcgcactac cctggtggcg ctggtggagt acgaaagccc
    1561  cgcggtgctc tcgcagatca tcgagctgga gtgccacggc gtgatcaccc agccgctcga
    1621  tgcccaccgg gtgctgcctg tgctggtatc ggcgcggcgc atcagcgagg aaatggcgaa
    1681  gctgaagcag aagaccgagc agctccagga ccgcatcgcc ggccaggccc ggatcaacca
    1741  ggccaaggtg ttgctgatgc agcgccatgg ctgggacgag cgcgaggcgc accagcacct
    1801  gtcgcgggaa gcgatgaagc ggcgcgagcc gatcctgaag atcgctcagg agttgctggg
    1861  aaacgagccg tccgcctgag cgatccgggc cgaccagaac aataacaaga ggggtatcgt
    1921  catcatgctg ggactggttc tgctgtacgt tggcgcggtg ctgtttctca atgccgtctg
    1981  gttgctgggc aagatcagcg gtcgggaggt ggcggtgatc aacttcctgg tcggcgtgct
    2041  gagcgcctgc gtcgcgttct acctgatctt ttccgcagca gccgggcagg gctcgctgaa
    2101  ggccggagcg ctgaccctgc tattcgcttt tacctatctg tgggtggccg ccaaccagtt
    2161  cctcgag
//
LOCUS       PAAMIS                  1130 bp    DNA     linear   BCT 10-AUG-1995
DEFINITION  P.aeruginosa (PAC1) amiS gene.
ACCESSION   X77161
VERSION     X77161.1  GI:473110
KEYWORDS    amiS gene.
SOURCE      Pseudomonas aeruginosa.
  ORGANISM  Pseudomonas aeruginosa
            Bacteria; Proteobacteria; gamma subdivision; Pseudomonadaceae;
            Pseudomonas.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Wilson,S.A., Wachira,S.M., Drew,R.E., Jones,D. and Pearl,L.H.
  TITLE     Identification of a putative ABC transporter in the Pseudomonas
            aeruginosa amidase operon
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Wilson,S.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-1994) S.A. Wilson, University College London,
            Dept of Biochemistry, Gower Street, London WC1E 6BT, UK
REFERENCE   3  (bases 1 to 1130)
  AUTHORS   Wilson,S.A., Williams,R.J., Pearl,L.H. and Drew,R.E.
  TITLE     Identification of two new genes in the Pseudomonas aeruginosa
            amidase operon, encoding an ATPase (AmiB) and a putative integral
            membrane protein (AmiS)
  JOURNAL   J. Biol. Chem. 270 (32), 18818-18824 (1995)
  MEDLINE   95370186
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Pseudomonas aeruginosa"
                     /strain="PAC1"
                     /db_xref="taxon:287"
                     /clone="plasmid pAS20"
     gene            61..576
                     /gene="amiS"
     CDS             61..576
                     /gene="amiS"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="CAA54406.1"
                     /db_xref="GI:473111"
                     /db_xref="SWISS-PROT:Q51417"
                     /translation="MLGLVLLYVGAVLFLNAVWLLGKISGREVAVINFLVGVLSACVA
                     FYLIFSAAAGQGSLKAGALTLLFAFTYLWVAANQFLEVDGKGLGWFCLFVSLTACTVA
                     IESFAGASGPFGLWNAVNWTVWALLWFCFFLLLGLSRGIQKPVAYLTLASAIFTAWLP
                     GLLLLGQVLKA"
BASE COUNT      167 a    335 c    377 g    251 t
ORIGIN
       1  gagccgtccg cctgagcgat ccgggccgac cagaacaata acaagagggg tatcgtcatc
      61  atgctgggac tggttctgct gtacgttggc gcggtgctgt ttctcaatgc cgtctggttg
     121  ctgggcaaga tcagcggtcg ggaggtggcg gtgatcaact tcctggtcgg cgtgctgagc
     181  gcctgcgtcg cgttctacct gatcttttcc gcagcagccg ggcagggctc gctgaaggcc
     241  ggagcgctga ccctgctatt cgcttttacc tatctgtggg tggccgccaa ccagttcctc
     301  gaggtggacg gcaagggcct cggctggttc tgcctgttcg tcagcctcac cgcctgcacc
     361  gtggcgatcg agtcgttcgc cggcgccagt ggtccgttcg gcctgtggaa cgcggtcaac
     421  tggacagtct gggcgttgct ctggttctgt ttcttcctgc tgctggggct gtcccgcggc
     481  atccagaagc cggtggccta cctgaccctg gccagcgcca tattcaccgc ctggttgccc
     541  ggcctgctgc tgctcggaca ggtgctcaag gcatagcagg aagtcggaaa gggatgacgg
     601  cttgccgcca tcccgtccct tccgaacgcc tagccgagcg gccagttgat caccacgacg
     661  gcgtcgttgt agtcgttgtc ggtgccgtct tcagagccga ccagggcgaa gttcagctcg
     721  ttggtcagga ttacctgtgc cgagaccaga tccgaggggc ggccgttgac gctgacctgg
     781  acctgtacct tgccactgct gccggagttg agcacctggg tgccgatgac ggcgttattg
     841  gtgctttgcc cgctgaaggt cgcggccgtg ctcgttgttg accagcacgt tcaccgtctg
     901  ggttccggac gagttggcga aggcggtgac gccggaacct ggttgttggc gggaagggtg
     961  aacactcctt gtggttgcca tggtggtatc tccactgaat acctggcccc ttccttttca
    1021  ggcagccgtc tggcgcgcgg tatggcgtgt cgggagaaat ccgcagtcct tggcggcagg
    1081  cgatgcgcag gcaggaagga cgcatcgttc agccaatcta cgccgtcgac
//
