LOCUS       H45989                   495 bp    mRNA    linear   EST 31-JUL-1995
DEFINITION  yo13c02.s1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone
            IMAGE:177794 3', mRNA sequence.
ACCESSION   H45989
VERSION     H45989.1  GI:922041
KEYWORDS    EST.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M., Holman
            ,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M., Parsons,J.,
            Rifkin,L., Rohlfing,T., Soares,M., Tan,F., Trevaskis,E., Waterston
            ,R., Williamson,A., Wohldmann,P. and Wilson,R.
  TITLE     The WashU-Merck EST Project
  JOURNAL   Unpublished (1995)
COMMENT     Contact: Wilson RK
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: est@watson.wustl.edu
            Insert Size: 544
            High quality sequence stops: 265
            Source: IMAGE Consortium, LLNL
            This clone is available royalty-free through LLNL ; contact the
            IMAGE Consortium (info@image.llnl.gov) for further information.
            Possible reversed clone: polyT not found
            Insert Length: 544   Std Error: 0.00
            Seq primer: SP6
            High quality sequence stop: 265.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Homo sapiens"
                     /db_xref="GDB:3839990"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:177794"
                     /clone_lib="Soares adult brain N2b5HB55Y"
                     /sex="Male"
                     /dev_stage="55-year old"
                     /lab_host="DH10B (ampicillin resistant)"
                     /note="Organ: brain; Vector: pT7T3D (Pharmacia) with a
                     modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
                     strand cDNA was primed with a Not I - oligo(dT) primer [5'
                     TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
                     double-stranded cDNA was size selected, ligated to Eco RI
                     adapters (Pharmacia), digested with Not I and cloned into
                     the Not I and Eco RI sites of a modified pT7T3 vector
                     (Pharmacia). Library went through one round of
                     normalization to a Cot = 53. Library constructed by Bento
                     Soares and M.Fatima Bonaldo. The adult brain RNA was
                     provided by Dr. Donald H. Gilden. Tissue was acquired
                     17-18 hours after death which occurred in consequence of a
                     ruptured aortic aneurysm. RNA was prepared from a pool of
                     tissues representing the following areas of the brain:
                     frontal, parietal, temporal and occipital cortex from the
                     left and right hemispheres, subcortical white matter,
                     basal ganglia, thalamus, cerebellum, midbrain, pons and
                     medulla."
BASE COUNT       73 a    135 c    169 g    104 t     14 others
ORIGIN
       1  ccggnaagct cancttggac caccgactct cgantgnntc gccgcgggag ccggntggan
      61  aacctgagcg ggactggnag aaggagcaga gggaggcagc acccggcgtg acggnagtgt
     121  gtggggcact caggccttcc gcagtgtcat ctgccacacg gaaggcacgg ccacgggcag
     181  gggggtctat gatcttctgc atgcccagct ggcatggccc cacgtagagt ggnntggcgt
     241  ctcggtgctg gtcagcgaca cgttgtcctg gctgggcagg tccagctccc ggaggacctg
     301  gggcttcagc ttcccgtagc gctggctgca gtgacggatg ctcttgcgct gccatttctg
     361  ggtgctgtca ctgtccttgc tcactccaaa ccagttcggc ggtccccctg cggatggtct
     421  gtgttgatgg acgtttgggc tttgcagcac cggccgccga gttcatggtn gggtnaagag
     481  atttgggttt tttcn
//
