EMBOSS: coderet

| ## EMBOSS: coderet

# Program coderet

## Function

Extract CDS, mRNA and translations from feature tables

## Description

The feature table of sequence database entries often have sections like this:

This specifies that the coding sequence for the gene is constructed by joining several sections of code, many of which are in other entries in this database.

    FT   CDS             join(U21925.1:818..987,U21926.1:258..420,
    FT                   U21927.1:428..520,U21928.1:196..336,U21929.1:279..415,
    FT                   U21930.1:895..1014,516..708)

or:

This specifies that the messenger RNA sequence for the gene is constructed by joining several sections of code, many of which are in other entries in this database.

    FT   mRNA            join(M88628.1:1006..1318,M88629.1:221..342,
    FT                   M88630.1:101..223,M88631.1:46..258,M88632.1:104..172,
    FT                   M88633.1:387..503,M88634.1:51..272,M88635.1:303..564,
    FT                   M88635.1:849..1020,M88636.1:282..375,M88637.1:39..253,
    FT                   M88638.1:91..241,M88639.1:168..377,M88640.1:627..3732,
    FT                   M88641.1:158..311,M88642.1:1051..1263,M88642.1:1550..1778,
    FT                   M88642.1:1986..2168,M88642.1:3904..4020,
    FT                   M88642.1:4627..4698,M88643.1:39..124,M88644.1:42..197,
    FT                   M88645.1:542..686,M88646.1:75..223,M88647.1:109..285,
    FT                   253..2211)

or:

This specifies that the translation of the coding region is as follows.

    FT                   /translation="MAQDSVDLSCDYQFWMQKLSVWDQASTLETQQDTCLHVAQFQEFL
    FT                   RKMYEALKEMDSNTVIERFPTIGQLLAKACWNPFILAYDESQKILIWCLCCLINKEPQN
    FT                   SGQSKLNSWIQGVLSHILSALRFDKEVALFTQGLGYAPIDYYPGLLKNMVLSLASELRE
    FT                   NHLNGFNTQRRMAPERVASLSRVCVPLITLTDVDPLVEALLICHGREPQEILQPEFFEA
    FT                   VNEAILLKKISLPMSAVVCLWLRHLPSLEKAMLHLFEKLISSERNCLRRIECFIKDSSL
    FT                   PQAACHPAIFRVDEMFRCALLETDGALEIIATIQVFTQCFVEALEKASKQLRFALKTYF
    FT                   PYTSPSLAMVLLQDPQDIPRGHWLQTLKHISELLREAVEDQTHGSCGGPFESWFLFIHF
    FT                   GGWAEMVAEQLLMSAAEPPTALLWLLAFYYGPRDGRQQRAQTMVQVKAVLGHLLAMSRS
    FT                   SSLSAQDLQTVAGQGTDTDLRAPAQQLIRHLLLNFLLWAPGGHTIAWDVITLMAHTAEI
    FT                   THEIIGFLDQTLYRWNRLGIESPRSEKLARELLKELRTQV"

It is often a tedious and error-prone job to extract the several sections of these sequences from the database entries and to join them together to construct the indicated finished sequence.

coderet does this job for you.

You specify the sequence containing the feature table and it extracts the required CDS, mRNA and/or protein sequences specified by this feature table. If any sequences are in other entries of that database, they are automatically fetched and incorporated correctly into the final sequence.

The translations are not made from the coding sequence, they are extracted directly from the translation sequence held in the feature table.

## Usage

Here is a sample session with coderet:

The EMBL sequence X03487's feature table is as follows:

    FH   Key             Location/Qualifiers
    FH
    FT   source          1..512
    FT                   /db_xref="taxon:9606"
    FT                   /organism="Homo sapiens"
    FT   misc_feature    65..70
    FT                   /note="GGGCGG box"
    FT   misc_feature    103..108
    FT                   /note="GGGCGG box"
    FT   misc_feature    126..131
    FT                   /note="GGGCGG box"
    FT   promoter        150..154
    FT                   /note="put. TATA box"
    FT   mRNA            179..500
    FT                   /note="exon 1"
    FT   CDS             join(387..500,X03488.1:50..196,X03488.1:453..578,
    FT                   X03488.1:674..838)
    FT                   /db_xref="SWISS-PROT:P02794"
    FT                   /label=apoh_cds
    FT                   /product="apoferritin H subunit"
    FT                   /protein_id="CAA27205.1"
    FT                   /translation="MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRD
    FT                   DVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECA
    FT                   LHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPE
    FT                   SGLAEYLFDKHTLGDSDNES"
    FT   intron          501..>512
    FT                   /note="intron I"

This means that the mRNA sequence is from position 179 to 500 in this sequence.
The coding sequence is formed by joining the region from 387 to 500 in this sequence together with regions from two other entries in this database: X03488 and X03488.
The translation of the coding sequence is also given.

To extract all of the CDS, mRNA and the protein translations, type:

    % coderet
    Extract CDS, mRNA and translations from feature tables
    Input sequence(s): embl:X03487
    Output sequence [hsferg1.fasta]:

The result is as follows:

    >x03487_cds_1
    atgacgaccgcgtccacctcgcaggtgcgccagaactaccaccaggactcagaggccgcc
    atcaaccgccagatcaacctggagctctacgcctcctacgtttacctgtccatgtcttac
    tactttgaccgcgatgatgtggctttgaagaactttgccaaatactttcttcaccaatct
    catgaggagagggaacatgctgagaaactgatgaagctgcagaaccaacgaggtggccga
    atcttccttcaggatatcaagaaaccagactgtgatgactgggagagcgggctgaatgca
    atggagtgtgcattacatttggaaaaaaatgtgaatcagtcactactggaactgcacaaa
    ctggccactgacaaaaatgacccccatttgtgtgacttcattgagacacattacctgaat
    gagcaggtgaaagccatcaaagaattgggtgaccacgtgaccaacttgcgcaagatggga
    gcgcccgaatctggcttggcggaatatctctttgacaagcacaccctgggagacagtgat
    aatgaaagctaa
    >x03487_mrna_1
    cagacgttcttcgccgagagtcgtcggggtttcctgcttcaacagtgcttggacggaacc
    cggcgctcgttccccaccccggccggccgcccatagccagccctccgtcgacctcttcac
    cgcaccctcggactgccccaaggcccccgccgccgctccagcgccgcgcagccaccgccg
    ccgccgccgcctctccttagtcgccgccatgacgaccgcgtccacctcgcaggtgcgcca
    gaactaccaccaggactcagaggccgccatcaaccgccagatcaacctggagctctacgc
    ctcctacgtttacctgtccatg
    >x03487_pro_1
    MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQS
    HEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHK
    LATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSD
    NES

Note that there is a mixture of nucleic and protein sequences in this file

To only extract the mRNA sequence, type:

    % coderet -nocds -notranslation
    Extract CDS, mRNA and translations from feature tables
    Input sequence(s): embl:X03487
    Output sequence [hsferg1.fasta]:

## Command line arguments

       Mandatory qualifiers:
      [-seqall]            seqall     Sequence database USA
      [-seqout]            seqout     Output sequence USA

       Optional qualifiers: (none)
       Advanced qualifiers:
       -[no]cds            bool       Extract CDS sequences
       -[no]mrna           bool       Extract mrna sequences
       -[no]translation    bool       Extract translated sequences

       General qualifiers:
      -help                bool       report command line options. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose

Mandatory qualifiers | Allowed values | Default
--- | --- | ---
[-seqall] (Parameter 1) | Sequence database USA | Readable sequence(s) | Required
[-seqout] (Parameter 2) | Output sequence USA | Writeable sequence | <sequence>.format
Optional qualifiers | Allowed values | Default
(none)
Advanced qualifiers | Allowed values | Default
-[no]cds | Extract CDS sequences | Yes/No | Yes
-[no]mrna | Extract mrna sequences | Yes/No | Yes
-[no]translation | Extract translated sequences | Yes/No | Yes

## Input file format

USA of one or more nucleic sequences having CDS, mRNA or translation headings in their feature tables.

## Output file format

The output is a sequence file containing any CDS, mRNA and protein translation sequences as specified by the feature table of the sequence(s).

One or more of CDS, mRNA, translation can be excluded from the output by using the appropriate qualifiers to the program (i.e. -nocds, etc.)

The ID names of the output sequences are constructed from the name of the input sequence, the type of feature being output (i.e. cds, mrna, pro) and a unique ordinal number for this type to distinguish it from others in this sequence. The name, type and number of separated by underscore characters. Thus the second CDS feature in the sequence 'HSXYZ' would be named 'HSXYZ_cds_2'.

The translations are not made from the coding sequence, they are extracted directly from the translation sequence held in the feature table.

## Data files

None.

## Notes

The translations are not made from the coding sequence, they are extracted directly from the translation sequence held in the feature table.

## References

None.

## Warnings

None.

## Diagnostic Error Messages

None.

## Exit status

It always exits with status 0.

## Known bugs

None.

## See also

Program name | Description
--- | ---
backtranseq | Back translate a protein sequence
extractfeat | Extract features from a sequence
maskfeat | Mask off features of a sequence
plotorf | Plot potential open reading frames
prettyseq | Output sequence with translated ranges
remap | Display a sequence with restriction cut sites, translation etc
showfeat | Show features of a sequence
showorf | Pretty output of DNA translations
showseq | Display a sequence with features, translation etc
swissparse | Retrieves sequences from swissprot using keyword search
transeq | Translate nucleic acid sequences

## Author(s)

This application was written by Alan Bleasby (ableasby@hgmp.mrc.ac.uk)

## History

Written (Nov 2000) - Alan Bleasby.

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments