# Enzyme list:
# name sequence temperature heat buffer
# > denotes the 3' end of the left hand fragment
# < denotes the 3' end of the right hand fragment
# name sequence temperature heat buffer
# temperature - optimal temperature
# heat: * means enzyme is heat inactivated
#  	o means enzyme is not heat inactivated
# Example:
# AatII G<ACGT>C 37 * NEB4
# 
AaaI C>GGCC<G
AacI G>GATC<C
AaeI G>GATC<C
AagI AT>CG<AT
AaqI G>TGCA<C
AatI AGG><CCT
AatII G<ACGT>C
AbrI C>TCGA<G
AcaI TT>CG<AA
AcaII G>GATC<C
AcaIII TGC><GCA
AcaIV GG><CC
AccI GT>mk<AC
AccII CG><CG
AccIII T>CCGG<A
Acc113I AGT><ACT
Acc16I TGC><GCA
Acc38I >CCwGG<
Acc65I G>GTAC<C
AccB1I G>GyrC<C
AccB2I r<GCGC>y
AccB7I CCAn<nnn>nTGG
AccEBI G>GATC<C
AceI G>CwG<C
AceII G<CTAG>C
AceIII CAGCTCnnnnnnn>nnnn<
AciI C>CG<C
AclI AA>CG<TT
AclNI A>CTAG<T
AclWI GGATCnnnn>n<
AcpI TT>CG<AA
AcpII CCAn<nnn>nTGG
AcrI C>yCGr<G
AcrII G>GTnAC<C
AcsI r>AATT<y
Acs1371I G>TCGA<C
Acs1372I G>TCGA<C
Acs1373I G>TCGA<C
Acs1421I G>TCGA<C
Acs1422I G>TCGA<C
AcyI Gr>CG<yC
AeuI >CCwGG<
AfaI GT><AC
Afa22MI CC<AT>GG
Afa24RI GCC><GGC
AfeI AGC><GCT
AflI G>GwC<C
AflII C>TTAA<G
AflIII A>CryG<T
AflIV AGT><ACT
AgeI A>CCGG<T
AhaI CC>s<GG
AhaII Gr>CG<yC
AhaIII TTT><AAA
AhaB1I C>CnG<G
AhaB8I G>GATC<C
AhdI GACnn<n>nnGTC
AhyI C>CCGG<G
AhyAI C>TCGA<G
AinI C<TGCA>G
AinII G>GATC<C
AitI AGC><GCT
AitII r>GATC<y
AitAI r>GATC<y
AjoI C<TGCA>G
AliI G>GATC<C
Ali2882I C<TCGA>G
Ali12257I G>GATC<C
Ali12258I G>GATC<C
AliAJI C<TCGA>G
AluI AG><CT
AlwI GGATCnnnn>n<
Alw21I G<wGCw>C
Alw26I GTCTCn>nnnn<
Alw44I G>TGCA<C
AlwNI CAG<nnn>CTG
AlwXI GCAGCnnnnnnnn>nnnn<
AmaI TCG><CGA
Ama67I C>yCGr<G
Ama87I C>yCGr<G
AmeI G>TGCA<C
AmeII GCC><GGC
AniMI GCC><GGC
AocI CC>TnA<GG
AocII G<dGCh>C
AorI CC>w<GG
Aor13HI T>CCGG<A
Aor51HI AGC><GCT
AosI TGC><GCA
AosII Gr>CG<yC
AosIII CC<GC>GG
ApaI G<GGCC>C
ApaBI GCA<nnnnn>TGC
ApaCI G>GATC<C
ApaLI G>TGCA<C
ApaORI CC>w<GG
ApeI A>CGCG<T
ApeAI GCC><GGC
ApiI C<TGCA>G
ApoI r>AATT<y
AprI GCC><GGC
ApuI G>GnC<C
Apu16I AT>CG<AT
ApyI CC>w<GG
AquI C>yCGr<G
AscI GG>CGCG<CC
AseI AT>TA<AT
AseII CC>s<GG
AsnI AT>TA<AT
AspI GACn>n<nGTC
Asp1I CC>s<GG
Asp10HI TT>CG<AA
Asp10HII CCAn<nnn>nTGG
Asp14HI r>GATC<y
Asp15I C>TCGA<G
Asp16HI GT><AC
Asp17I r>GATC<y
Asp17HI GT><AC
Asp18HI GT><AC
Asp1HI r>GATC<y
Asp2HI >CCwGG<
Asp21HI r>GATC<y
Asp22I r>GATC<y
Asp26HI GAATG<Cn>
Asp27HI GAATG<Cn>
Asp29HI GT><AC
Asp32HI CC<GC>GG
Asp35HI GAATG<Cn>
Asp36I C<TGCA>G
Asp36HI GAATG<Cn>
Asp40HI GAATG<Cn>
Asp47I C>TCGA<G
Asp50HI GAATG<Cn>
Asp52I A>AGCT<T
Asp5HI G>CATG<C
Asp78I AGG><CCT
Asp697I G>GwC<C
Asp6HI r>GATC<y
Asp700I GAAnn><nnTTC
Asp703I C>TCGA<G
Asp707I AT>CG<AT
Asp708I C<TGCA>G
Asp713I C<TGCA>G
Asp718I G>GTAC<C
Asp742I GG><CC
Asp745I G>GwC<C
Asp748I C>CG<G
Asp763I AGT><ACT
Asp8HI r>GATC<y
Asp3065I A>AGCT<T
AspAI G>GTnAC<C
AspBI C>yCGr<G
AspBII G>GwC<C
AspCI C>yCGr<G
AspCII G>GwC<C
AspDI C>yCGr<G
AspDII G>GwC<C
AspEI GACnn<n>nnGTC
AspHI G<wGCw>C
AspJI G<ACGT>C
AspLEI G<CG>C
AspMDI >GATC<
AspNI GGn><nCC
AspS9I G>GnC<C
AspTI C<TGCA>G
AspTII G>GATC<C
AspTIII GG><CC
AstWI Gr>CG<yC
AsuI G>GnC<C
AsuII TT>CG<AA
AsuIII Gr>CG<yC
AteI C>CATG<G
AtsI GACn>n<nGTC
AtuII >CCwGG<
Atu1I >CCwGG<
Atu1II G>GATC<C
AtuBI >CCwGG<
AtuCI T>GATC<A
AvaI C>yCGr<G
AvaII G>GwC<C
AvaIII A<TGCA>T
AvcI G>GnC<C
AviI TT>CG<AA
AviII TGC><GCA
AvrI C>yCGr<G
AvrII C>CTAG<G
AvrBI GG><CC
AvrBII C>CTAG<G
AxyI CC>TnA<GG
BacI CC<GC>GG
Bac36I G>GnC<C
Bac465I CC<GC>GG
BadI C>TCGA<G
BalI TGG><CCA
Bal228I G>GnC<C
Bal475I GG><CC
Bal3006I GG><CC
BamFI G>GATC<C
BamHI G>GATC<C
BamKI G>GATC<C
BamNI G>GATC<C
BamNxI G>GwC<C
BanI G>GyrC<C
BanII G<rGCy>C
BanIII AT>CG<AT
BavI CAG><CTG
BavAI CAG><CTG
BavAII G>GnC<C
BavBI CAG><CTG
BavBII G>GnC<C
BavCI AT>CG<AT
BbeI G<GCGC>C
BbeAI GG>CG<CC
BbfI C>TCGA<G
Bbf7411I T>CCGG<A
BbiI C<TGCA>G
BbiII Gr>CG<yC
BbiIII C<TGCA>G
Bbi24I A>CGCG<T
BbrI A>AGCT<T
BbrAI A>AGCT<T
BbrPI CAC><GTG
BbsI GAAGACnn>nnnn<
BbuI G<CATG>C
BbvI GCAGCnnnnnnnn>nnnn<
BbvII GAAGACnn>nnnn<
Bbv12I G<wGCw>C
Bbv16II GAAGACnn>nnnn<
BbvAI GAAnn><nnTTC
BbvAII AT>CG<AT
BbvAIII T>CCGG<A
BbvBI G>GyrC<C
BcaI G<CG>C
Bca77I w>CCGG<w
Bca1259I G>GATC<C
BccI CC><ATC
Bce22I G>GnC<C
Bce71I GG><CC
Bce83I CTTGAGnnnnnnnnnnnnnn<nn>
Bce170I C<TGCA>G
Bce243I >GATC<
Bce751I G>GATC<C
Bce1247I GCnn<nnn>nnGC
BceFI CG><CG
BceRI CG><CG
BcefI ACGGCnnnnnnnnnnnn>n<
BcgI CGAnnnnnnTGCnnnnnnnnnn<nn>
Bci29I AT>CG<AT
BciBI AT>CG<AT
BciBII CC>w<GG
BclI T>GATC<A
BcmI AT>CG<AT
BcnI CC>s<GG
BcoI C>yCGr<G
Bco5I CTCTTCn>nnn<
Bco6I TGC><GCA
Bco27I C>CG<G
Bco33I GG><CC
Bco35I CTGGAGnnnnnnnnnnnnnn<nn>
Bco63I GATnn><nnATC
Bco102I T>GATC<A
Bco102II GAAGACnn>nnnn<
Bco116I CTCTTCn>nnn<
Bco118I r>CCGG<y
Bco163I C>TryA<G
Bco10278I G>GATC<C
BcoAI CAC><GTG
BcoKI CTCTTCn>nnn<
BcrI GGn><nCC
BctI ACGGCnnnnnnnnnnnn>n<
BdiI AT>CG<AT
BdiSI C>TryA<G
BepI CG><CG
BetI w>CCGG<w
BfaI C>TA<G
BfiI ACT><GGG
Bfi57I >GATC<
Bfi89I y>GGCC<r
Bfi458I GG><CC
BfmI C>TryA<G
BfrI C>TTAA<G
BfrAI AT>CG<AT
BfrBI ATG><CAT
BfrCI ATG><CAT
BglI GCCn<nnn>nGGC
BglII A>GATC<T
BheI GCC><GGC
BimI TT>CG<AA
Bim19I TT>CG<AA
Bim19II GG><CC
BinI GGATCnnnn>n<
BinSI >CCwGG<
BinSII GG>CG<CC
Bka1125I G<dGCh>C
Bla7920I T>CCGG<A
BliI GG><CC
Bli4I AT>CG<AT
Bli49I GGTCTCn>nnnn<
Bli86I AT>CG<AT
Bli736I GGTCTCn>nnnn<
BliHKI CC>TnA<GG
BliRI AT>CG<AT
BlnI C>CTAG<G
BlpI GC>TnA<GC
BluI C>TCGA<G
BluII GG><CC
BmaI CG<AT>CG
BmaAI CG<AT>CG
BmaBI CG<AT>CG
BmaCI CG<AT>CG
BmaDI CG<AT>CG
Bme12I >GATC<
Bme18I C>CwG<G
Bme142I rGC><GCy
Bme216I G>GwC<C
Bme1390I CC>n<GG
BmeBI C<TGCA>G
BmeTI T>GATC<A
BmgI GkG><CCC
BmyI G<dGCh>C
BnaI G>GATC<C
BpeI A>AGCT<T
BpiI GAAGACnn>nnnn<
BplI GAGnn><nnnCTC
BpmI CTGGAGnnnnnnnnnnnnnn<nn>
BpuI G<rGCy>C
Bpu10I CC>TnA<GC
Bpu1102I GC>TnA<GC
Bpu14I TT>CG<AA
Bpu95I CG><CG
Bpu1268I CCTnn>n<nnAGG
BpuAI GAAGACnn>nnnn<
BpuB5I C>GTAC<G
BsaI GGTCTCn>nnnn<
Bsa29I AT>CG<AT
BsaAI yAC><GTr
BsaBI GATnn><nnATC
BsaCI CC>n<GG
BsaDI G>GATC<C
BsaEI GGn><nCC
BsaFI C>TTAA<G
BsaGI G<wGCw>C
BsaHI Gr>CG<yC
BsaJI C>CnnG<G
BsaKI GTT><AAC
BsaLI AG><CT
BsaMI GAATG<Cn>
BsaNI >CCwGG<
BsaNII C<TGCA>G
BsaOI CG<ry>CG
BsaPI >GATC<
BsaQI C<TGCA>G
BsaRI GG><CC
BsaSI G>GnC<C
BsaTI TGC><GCA
BsaUI GCAGCnnnnnnnn>nnnn<
BsaVI GAAGACnn>nnnn<
BsaWI w>CCGG<w
BsaXI ACnnn><nnCTCC
BsaZI C>CG<G
BsbI CAA><CAC
BscI AT>CG<AT
Bsc4I CCnnn<n>nnnGG
Bsc91I GAAGACnnn>nnnn<
Bsc92I GAAGACnn>nnnn<
Bsc107I CCnn<nnn>nnGG
BscAI GCATCnnnn>nn<
BscBI GGn><nCC
BscCI GAATG<Cn>
BscDI C<TGCA>G
BscEI G>CGCG<C
BscFI >GATC<
BscGI CC><CGT
BscHI ACTG<Gn>
BscJI CCAn<nnnn>nTGG
BseI GG><CC
BseII GTT><AAC
Bse1I ACTG<Gn>
Bse118I r>CCGG<y
Bse15I C>yCGr<G
Bse16I CC>w<GG
Bse17I CC>w<GG
Bse21I CC>TnA<GG
Bse23I CCnn<nnn>nnGG
Bse24I CC>w<GG
Bse59I G>GTnAC<C
Bse634I r>CCGG<y
Bse64I G>GTnAC<C
Bse8I GATnn><nnATC
BseAI T>CCGG<A
BseBI CC>w<GG
BseCI AT>CG<AT
BseDI C>CnnG<G
BseHI A>AGCT<T
BseNI ACTG<Gn>
BsePI G>CGCG<C
BseQI GG><CC
BseRI GAGGAGnnnnnnnn<nn>
BseT9I G>GTnAC<C
BseT10I G>GTnAC<C
BseZI CTCTTCn>nnn<
BsgI GTGCAGnnnnnnnnnnnnnn<nn>
BshI GG><CC
Bsh1236I CG><CG
Bsh1285I CG<ry>CG
Bsh1365I GATnn><n
Bsh45I G<wGCw>C
BshAI GG><CC
BshBI GG><CC
BshCI GG><CC
BshDI GG><CC
BshEI GG><CC
BshFI GG><CC
BshGI CC>w<GG
BshHI AGT><ACT
BshKI G>GnC<C
BshLI GAT><ATC
BshMI C>CG<G
BshNI G>GyrC<C
BsiI C>TCGT<G
BsiAI GG><CC
BsiBI GATnn><nnATC
BsiCI TT>CG<AA
BsiDI GG><CC
BsiEI CG<ry>CG
BsiGI T>CCGG<A
BsiHI GG><CC
BsiHKAI G<wGCw>C
BsiKI G>GTnAC<C
BsiLI CC>w<GG
BsiMI T>CCGG<A
BsiOI T>CCGG<A
BsiQI T>GATC<A
BsiSI C>CG<G
BsiUI CC>w<GG
BsiVI CC>w<GG
BsiWI C>GTAC<G
BsiXI AT>CG<AT
BsiYI CCnn<nnn>nnGG
BsiZI G>GnC<C
BslI CCnn<nnn>nnGG
BsmI GAATG<Cn>
BsmAI GTCTCn>nnnn<
BsmBI CGTCTCn>nnnn<
BsmCI ACnnn><nnCTCC
BsmDI ACnnn><nnCTCC
BsmEI GAGTCnnnn>n<
BsmFI GGGACnnnnnnnnnn>nnnn<
BsmGI T>GTAAC<A
BsmGII A>AGCT<T
BsmHI r<GCGC>y
BsmNI GCATCnnnnn>nnnn<
BsmPI G<wGCw>C
BsmSI C>CwwG<G
BsmXI ACnnn><nnCTCC
BsmXII >GATC<
BsmYI CCnn<nnn>nnGG
BsoI CC>n<GG
BsoAI GAT><ATC
BsoBI C>yCGr<G
BsoCI G<dGCh>C
BsoDI C>GGCC<G
BsoEI CCTnn>n<nnAGG
BsoFI GC>n<GC
BsoGI >CCwGG<
BsoHI ACTG<Gn>
BsoJI GCCn<nnn>nGGC
BsoPI G>CGCG<C
BsoSI AGT><ACT
BspI >GATC<
Bsp100I G>GwC<C
Bsp103I >CCwGG<
Bsp105I >GATC<
Bsp106I AT>CG<AT
Bsp119I TT>CG<AA
Bsp12I CC<GC>GG
Bsp120I G>GGCC<C
Bsp1286I G<dGCh>C
Bsp13I T>CCGG<A
Bsp1407I T>GTAAC<A
Bsp143I >GATC<
Bsp143II r<GCGC>y
Bsp16I GAT><ATC
Bsp17I C<TGCA>G
Bsp1720I GC>TnA<GC
Bsp18I >GATC<
Bsp19I C>CATG<G
Bsp2I AT>CG<AT
Bsp21I r>CCGG<y
Bsp22I CTGGAGnnnnnnnnnnnnnn<nn>
Bsp24I GACnnnnnnTGGnnnnnnn<nnnnn>
Bsp28I CTGGAGnnnnnnnnnnnnnn<nn>
Bsp29I GGn><nCC
Bsp30I G>GATC<C
Bsp4I AT>CG<AT
Bsp43I C<TGCA>G
Bsp46I G>GATC<C
Bsp47I C>CG<G
Bsp48I C>CG<G
Bsp49I >GATC<
Bsp5I C>CG<G
Bsp50I CG><CG
Bsp51I >GATC<
Bsp52I >GATC<
Bsp53I CC>n<GG
Bsp54I >GATC<
Bsp55I CC>s<GG
Bsp56I >CCwGG<
Bsp57I >GATC<
Bsp58I >GATC<
Bsp59I >GATC<
Bsp6I GC>n<GC
Bsp6II CTGAAGnnnnnnnnnnnnnn<nn>
Bsp60I >GATC<
Bsp61I >GATC<
Bsp63I C<TGCA>G
Bsp64I >GATC<
Bsp65I >GATC<
Bsp66I >GATC<
Bsp67I >GATC<
Bsp68I TCG><CGA
Bsp7I CC>s<GG
Bsp70I CG><CG
Bsp71I G>GwC<C
Bsp72I >GATC<
Bsp73I CC>n<GG
Bsp74I >GATC<
Bsp76I >GATC<
Bsp78I C<TGCA>G
Bsp8I CC>s<GG
Bsp81I C<TGCA>G
Bsp82I TT>CG<AA
Bsp84I AT>CG<AT
Bsp87I CAC><GTG
Bsp9I >GATC<
Bsp91I >GATC<
Bsp92I C>TCGA<G
Bsp93I C<TGCA>G
Bsp98I G>GATC<C
BspAI >GATC<
BspCI CG<AT>CG
BspDI AT>CG<AT
BspEI T>CCGG<A
BspGI CTG><GAC
BspHI T>CATG<A
BspH614I CTGGAGnnnnnnnnnnnnnn<nn>
BspKI GG><CC
BspLU11I A>CATG<T
BspMI ACCTGCnnnn>nnnn<
BspMII T>CCGG<A
BspM90I GTA><TAC
BspWI GCnn<nnn>nnGC
BspXI AT>CG<AT
BsrI ACTG<Gn>
BsrBI GAG><CGG
BsrDI GCAATG<nn>
BsrFI r>CCGG<y
BsrGI T>GTAC<A
BssHII G>CGCG<C
BssSI C>TCGT<G
BssT1I C>CwwG<G
BstI G>GATC<C
Bst1I CC>w<GG
Bst1107I GTA><TAC
Bst38I CC>w<GG
Bst71I GCAGCnnnnnnnn>nnnn<
BstBI TT>CG<AA
BstEII G>GTnAC<C
BstNI CC>w<GG
BstOI CC>w<GG
BstPI G>GTnAC<C
BstUI CG><CG
BstVI C>TCGA<G
BstXI CCAn<nnnn>nTGG
BstYI r>GATC<y
BstZI C>GGCC<G
Bsu15I AT>CG<AT
Bsu23I T>CCGG<A
Bsu36I CC>TnA<GG
Bsu54I G>GnC<C
BsuRI GG><CC
Cac8I GCn><nGC
CauII CC>s<GG
CcrI C>TCGA<G
CelII GC>TnA<GC
CfoI G<CG>C
CfrI y>GGCC<r
Cfr10I r>CCGG<y
Cfr13I G>GnC<C
Cfr42I CC<GC>GG
Cfr9I C>CCGG<G
ClaI AT>CG<AT
CpoI CG>GwC<CG
CspI CG>GwC<CG
Csp45I TT>CG<AA
Csp6I G>TA<C
CviJI rG><Cy
CviRI TG><CA
CvnI CC>TnA<GG
DdeI C>TnA<G
DpnI GA><TC
DpnII >GATC<
DraI TTT><AAA
DraII rG>GnC<Cy
DraIII CAC<nnn>GTG
DrdI GACnn<nn>nnGTC
DrdII GAA><CCA
DsaI C>CryG<G
DsaV >CCnGG<
EaeI y>GGCC<r
EagI C>GGCC<G
Eam1104I CTCTTCn>nnn<
Eam1105I GACnn<n>nnGTC
EarI CTCTTCn>nnn<
EciI TCC><GCC
EclXI C>GGCC<G
Ecl136II GAG><CTC
Eco105I TAC><GTA
Eco130I C>CwwG<G
Eco147I AGG><CCT
Eco24I G<rGCy>C
Eco31I GGTCTCn>nnnn<
Eco32I GAT><ATC
Eco47I G>GwCC<
Eco47III AGC><GCT
Eco52I C>GGCC<G
Eco57I CTGAAGnnnnnnnnnnnnnn<nn>
Eco64I G>GyrC<C
Eco72I CAC><GTG
Eco81I CC>TnA<GG
Eco88I C>yCGr<G
Eco91I GG>TnA<CC
EcoICRI GAG><CTC
EcoNI CCTnn>n<nnAGG
EcoO65I G>GTnAC<C
EcoO109I rG>GnC<Cy
EcoRI G>AATT<C
EcoRII >CCwGG<
EcoRV GAT><ATC
EcoT14I C>CwwG<G
EcoT22I A<TGCA>T
EheI GGC><GCC
ErhI C>CwwG<G
EspI GC>TnA<GC
Esp3I CGTCTCn>nnnn<
Esp4I C>TTAA<G
FauI CCCGCnnnn>nn<
FbaI T>GATC<A
FblI GT>mk<AC
FdiII TGC><GCA
FinI GGGACnnnnnnnnnn>nnnn<
Fnu4HI GC>n<GC
FnuDII CG><CG
FokI GGATGnnnnnnnnn>nnnn<
FseI GG<CCGG>CC
FspI TGC><GCA
GdiII y>GGCC<G
GsuI CTGGAGnnnnnnnnnnnnnn<nn>
HaeI wGG><CCw
HaeII r<GCGC>y
HaeIII GG><CC
HapII C>CG<G
HgaI GACGCnnnnn>nnnnn<
HgiAI G<wGCw>C
HgiCI G>GyrC<C
HgiEII ACCnnn><nnnGGT
HgiJII G<rGCy>C
HhaI G<CG>C
Hin1I Gr>CG<yC
Hin4I GAbnn><nnnvTC
Hin6I G>CG<C
HincII GTy><rAC
HindII GTy><rAC
HindIII A>AGCT<T
HinfI G>AnT<C
HinPI G>CG<C
HinP1I G>CG<C
HpaI GTT><AAC
HpaII C>CG<G
HphI GGTGAnnnnnnn<n>
I-PpoI CTCTCT<TAA>GGTAGC
KasI G>GCGC<C
KpnI G<GTAC>C
Kpn2I T>CCGG<A
Kpn378I CC<GC>GG
KspI CC<GC>GG
Ksp22I T>GATC<A
Ksp632I CTCTTCn>nnn<
Kzo9I >GATC<
LspI TT>CG<AA
MaeI C>TA<G
MaeII A>CG<T
MaeIII >GTnAC<
MamI GATnn><nnATC
MboI >GATC<
MboII GAAGAnnnnnnn<n>
McrI CG<ry>CG
MflI r>GATC<y
MluI A>CGCG<T
MluTW11I GCC><GGC
Mly113I GG>CG<CC
MmeI TCCrACnnnnnnnnnnnnnnnnnn<nn>
MnlI CCTCnnnnnnn><
Mph1103I A<TGCA>T
Mph17I G>TATA<C
MraI CC<GC>GG
MroI T>CCGG<A
MscI TGG><CCA
MseI T>TA<A
MslI CAynn><nnrTG
MspI C>CG<G
Msp20I TGG><CCA
MspA1I CmG><CkG
MstI TGC><GCA
MstII CC>TnA<GG
MunI C>AATT<G
MvaI CC>w<GG
MvnI CG><CG
MwoI GCnn<nnn>nnGC
NaeI GCC><GGC
NarI GG>CG<CC
NciI CC>s<GG
NcoI C>CATG<G
NdeI CA>TA<TG
NdeII >GATC<
NgoAIV G>CCGG<C
NgoMI G>CCGG<C
NheI G>CTAG<C
NlaIII <CATG>
NlaIV GGn><nCC
NotI GC>GGCC<GC
NruI TCG><CGA
NsiI A<TGCA>T
NspI r<CATG>y
NspII G<dGCh>C
NspIII C>yCGr<G
NspIV G>GnC<C
NspV TT>CG<AA
Nsp7524I r<CATG>y
NspBII CmG><CkG
NspHI r<CATG>y
NunII GG>CG<CC
PacI TTA<AT>TAA
PaeI G<CATG>C
PaeR7I C>TCGA<G
PalI GG><CC
Pfl1108I TCG><TAG
Pfl23II C>GTAC<G
PflMI CCAn<nnn>nTGG
PinAI A>CCGG<T
PleI GAGTCnnnn>n<
Ple19I CG<AT>CG
PleT12I r>GATC<y
PmaCI CAC><GTG
PmeI GTTT><AAAC
Pme55I AGG><CCT
PmlI CAC><GTG
Ppu10I A>TGCA<T
PpuMI rG>GwC<Cy
PshAI GACnn><nnGTC
Psp124BI G<AGCT>C
Psp1406I AA>CG<TT
Psp5II rG>GwC<Cy
PspAI C>CCGG<G
PssI rG<GnC>Cy
PstI C<TGCA>G
PvuI CG<AT>CG
PvuII CAG><CTG
RcaI T>CATG<A
RleAI CCCACAnnnnnnnnn<nnn>
RmaI C>TA<G
RsaI GT><AC
RsrII CG>GwC<CG
RtrI G>TCGA<C
SacI G<AGCT>C
SacII CC<GC>GG
SalI G>TCGA<C
SalGI G>TCGA<C
SanDI GG>GwC<CC
SapI GCTCTTCn>nnn<
SauI CC>TnA<GG
Sau96I G>GnC<C
Sau3AI >GATC<
ScaI AGT><ACT
ScrFI CC>n<GG
SduI G<dGCh>C
SecIC>CnnG<G
SexAI A>CCwGG<T
SfaNI GCATCnnnnn>nnnn<
SfcI C>TryA<G
SfeI C>TryA<C
SfiI GGCCn<nnn>nGGCC
Sfr274I C>TCGA<G
Sfr303I CC<GC>GG
SfuI TT>CG<AA
SgfI GCG<AT>CGC
SgrAI Cr>CCGG<yG
SinI G>GwC<C
SlaI C>TCGA<G
SmaI CCC><GGG
SnaBI TAC><GTA
SnoI G>TGCA<C
SpeI A>CTAG<T
SphI G>CATG<C
SplI C>GTAC<G
SpoI TCG><CGA
SrfI GCCC><GGGC
Sse8387I CC<TGCA>GG
Sse8647I AG>GwC<CT
SspI AAT><ATT
SstI G<AGCT>C
SstII CC<GC>GG
StuI AGG><CCT
StyI C>CwwG<G
SunI C>GTAC<G
SwaI ATTT><AAAT
TaqI T>CG<A
TaqII CACCGAnnnnnnnnn<nn>
TauI GC><sGC
TfiI G>AwT<C
ThaI CG><CG
Tru9I T>TA<A
TseI GC><wGC
Tsp45I >GTsAC<
Tsp509I >AATT<
Tth111I GACn>n<nGTC
Tth111II CAArCAnnnnnnnnn<nn>
TthHB8I T>CG<A
UbaCI wGT><ACw
Van91I CCAn<nnn>nTGG
Vha464I C>TTAA<G
VneI G>TGCA<C
VneII GT>CCGG<AC
VspI AT>TA<AT
XbaI T>CTAG<A
XcmI CCAnnnn<n>nnnnTGG
XcyI C>CCGG<G
XhoI C>TCGA<G
XhoII r>GATC<y
XmaI C>CCGG<G
XmaIII C>GGCC<G
XmnI GAAnn><nnTTC
XorII CG<AT>CG
Zsp2I A<TGC>T
